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規(guī)格

規(guī)格	16樣本(產(chǎn)品加服務(wù))、64樣本(產(chǎn)品加服務(wù))、32樣本(產(chǎn)品加服務(wù))、16樣本、32樣本、64樣本
樣本類型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
原理方法	Sandwich-based
檢測(cè)方法	Fluorescence Laser Scanner
定量與否	Semi-Quantitative
載體	Glass Slide
適用種屬	Mouse
檢測(cè)靶標(biāo)	IFN-gamma, TNF alpha, bFGF, Decorin, IGF-1, IL-10, IL-13, IL-15, IL-17A, IL-2, IL-21, IL-4, LIF, VEGF-A, Cystatin C, Galectin-3, GM-CSF, HGF, IGFBP-2, IL-6, Nope, RAGE, Resistin, IL-5, IL-16, ACE-2, FGF-6, IGFBP-3, IL-12 p70, Leptin, Leptin R, M-CSF, Oncostatin M, PDGF-AA, TGF beta 1, Clusterin, CXCL16, EGF, GCSF, uPAR, VEGF-D, VEGFR2, VEGFR3, IGF-2, IGFBP-5, IGFBP-6, Activin A, ADAM-9, ALK-1, Alpha-fetoprotein, Amphiregulin, Artemin, AXL, C5a, CA9, CD14, CD200, CD45, CEACAM-1, Chemerin, COCO, Cripto-1, Cystatin B, DAN, DKK-1, DLL1, DLL4, Dtk, E-Cadherin, E-Selectin, EDAR, Endocan, EphA5, EphA8, EphB2, EphB4, EphB6, Epiregulin, Erythropoietin R, Fetuin A, FGF-21, FGF-4, FLRG, Flt-3 Ligand, Follistatin, Frizzled-1, Frizzled-4, Galectin-1, Galectin-7, Gas 1, GDF-3, Gremlin-1, HAI-1, IFN-gamma R1, IL-17B, IL-17C, IL-17F, IL-20, IL-22, IL-23, IL-3, IL-31, IL-34, IL-7, IL-9, Kallikrein 7, Kremen-1, Kremen-2, Layilin, LDL R, LIMPII, LOX-1, Marapsin, MBL-2, MFG-E8, MIP-2, MMP-10, MMP-2, MMP-3, Neprilysin, Neurturin, P-Cadherin, P-Selectin, PD-1, PDGF R beta, Persephin, Podocalyxin, Pro-MMP-9, Progranulin, Prolactin, Prostasin, Ret, sFRP-3, Syndecan-1, Syndecan-3, TGF beta R2, TLR2, TREM-1, TSLP, TSLP R, VEGF-B, VEGFR1, STC-2, Ameloblastin, Contactin-6, ASAHL, B7-H4, CA12, Cadherin-4, CD2, CD28, CD4, CD6, CD99-L2, CDCP1, CXADR, Ephrin-A4, Granzyme B, Matrilin-2, Matrilin-3, PLA2G2A, Relaxin-1, RGM-B, ROBO3, Semaphorin 3C, Semaphorin 4C, Semaphorin 4G, SorCS2, TFPI-2, TREM-2, Cathepsin H, Meprin alpha, Periostin, SP-D, SEMA3F, Slit2, Wnt-2b, Protocadherin-12, Chordin, IL-12 p40/p70, TIGIT, TRKC, CDNF, Gas 6, GPVI, CD69, Podoplanin, CLEC9A, Laminin alpha 4, TROP-2, Neurocan, Semaphorin 6C, DNER, Epigen, IL-17RA, IL-17RB, IL-23R, LIX, Olfactomedin-1, CFVII, MAdCAM-1, MCP-5, MIP-1 gamma, PLGF-2, MESDC2, NgR, PILR-beta, Plexin A1, Plexin C1, Reg2, Semaphorin 4F, SMOC-1, Tryptase-5, CCL6, H60, Limitin, Meteorin, Renin 1, Tryptase epsilon, IFN-gamma R2, Nephrin, Ephrin-A2, CD32A, Contactin-4 / BIG-2, Eotaxin-1 / CCL11, Fas Ligand / TNFSF6, MIP-1 alpha / CCL3, RANTES / CCL5, CTLA-4 / CD152, BLC / CXCL13, IL-33 / IL-1F11, TNF R1 / TNFRSF1A, IL-1 beta / IL-1F2, CD40 Ligand / TNFSF5, MIG / CXCL9, MIP-1 beta / CCL4, ADAM-15, ADP-Ribosyl Cyclase 2 / BST-1 / CD157, Angiopoietin-like protein 3, Angiopoietin-like protein 7, ASAM / ACAM, Carbonic Anhydrase XIV / CA14, Carbonic Anhydrase IV / CA4, CD27 / TNFRSF7, CD39L3 / ENTPD3, VE-Cadherin / CDH5, CHL-1 / L1CAM-2, Chordin-like 2 / CHRDL2, CRACC / SLAMF7, CD39 / ENTPD1, Follistatin-like 1 / FSTL1, GFR alpha-3 / GDNF R alpha-3, IGSF8 / CD316, IL-10R beta, IL-11R alpha, IL-15R alpha, IL-17RC, IL-1R-1 / IL-1RT-1 / IL-1R alpha / CD121a / p80, IL-1R-2 / IL-1RT-2 / IL-1R beta / CD121b, IL-20R beta, IL-21R, IL-2R alpha, IL-36 beta / IL-1F8, LRPAP / RAP, LRRC32 / GARP, PD-L2 / B7-DC, Siglec-3 / CD33, SR-AI / MSR, TGF beta R1 / ALK 5, CD90 / Thy1, BAFF R / TNFRSF13C, Thrombopoietin R / Tpo R, TRANCE / TNFSF11, SCF / KITLG, Angiopoietin-3 / ANGPTL1, CD86 / B7-2, B7-H3 / CD276, CD44 / Heparan Sulfate Proteoglycan, BRAK / CXCL14, FGF-10 / KGF-2, IL-18BPc, IL-1R-4 / ST2 / IL-33R, IL-3R beta, JAM-C / CD323, Klotho beta / BKL, LAMP1 / CD107a, LIGHT / TNFSF14, RGM-C / Hemojuvelin, Siglec-12 / Siglec-E / Siglec-L1, TIM-3 / KIM-3, TNF R2 / TNFRSF1B, 4-1BB Ligand / TNFSF9, IL-1 alpha / IL-1F1, TWEAK / TNFSF12, CD36 / SR-B3, CD40 / TNFRSF5, Endoglin / CD105, TROP1 / EpCAM, GITR / TNFRSF18, IL-7R alpha / CD127, JAM-A / CD321 / F11R, Lymphotoxin beta R / TNF RIII / TNFRSF3, SLAM / CD150, IL-1ra / IL-1RN / IL-1 F3 / IRAP, MCP-1 / CCL2, CXCL1 / GRO alpha / KC / CINC-1, HGFR / MET, TWEAK R / TNFRSF12, Fas / TNFRSF6 / Apo-1, MIP-3 alpha / CCL20, Pentraxin-3 / TSG-14, 2B4 / CD244, 6Ckine / CCL21, ACE / CD143, Activin R1B / ALK-4, Adiponectin / ACRP30, B7-H2 / ICOS Ligand, Betacellulin / BTC, C1q R1 / CD93, C4.4A / LYPD3, MEC / CCL28, CD229 / SLAMF3, CD27 Ligand / TNFSF7, CD30 / TNFRSF8, CD30 Ligand / TNFSF8, CD48 / SLAMF2, CD80 / B7-1, CRP / C-Reactive Protein, Cardiotrophin-1 / CT-1, Dectin-2 / CLEC6A, DNAM-1 / CD226, Endoglycan / PODXL2, Eotaxin-2 / MPIF-2 / CCL24, Epimorphin / Syntaxin??2, FCRL1 / FcRH1, Flt-3 / Flk-2, Fractalkine / CX3CL1, GCSF R / CD114, GITR Ligand / TNFSF18, GPV / CD42d, I-309 / TCA-3 / CCL1, ICAM-1 / CD54, IL-17E / IL-25, IL-18R beta / IL-1R-7 / AcPL / IL-18RAcP, IL-1Rrp2 / IL-1R-6, IL-28A / IFN lambda 2, I-TAC / CXCL11, Lipocalin-2 / NGAL, L-Selectin / CD62L, Lymphotactin / XCL1, MDC / CCL22, MEPE / OF45, MIP-3 beta / CCL19, Nectin-2 / CD112, NGFR / TNFRSF16, NKp46 / NCR1 / CD335, NOV / CCN3, Osteoprotegerin / TNFRSF11B, Osteopontin / SPP1, Osteoadherin / Osteomodulin, Oncostatin M R beta / IL-31 R beta, Osteoactivin / GPNMB, OX40 / TNFRSF4, OX40 Ligand / TNFSF4, Platelet Factor 4 / CXCL4, RANK / TNFRSF11A, SCF R / CD117 / c-kit, SDF-1 alpha / CXCL12 alpha, gp130 / sgp130, Sonic Hedgehog N-Terminal / Shh-N, TACI / TNFRSF13B, TARC / CCL17, TECK / CCL25, Transglutaminase 2 / TGM2, TIM-1 / KIM-1, 4-1BB / TNFRSF9 / CD137, Thrombopoietin / TPO, TRAIL / TNFSF10, TRAIL R2 / TNFRSF10B / DR5, TREML1 / TLT-1, TROY / TNFRSF19, Trypsin 3 / PRSS3, VCAM-1 / CD106, ADAMTS-1 / METH1, Lungkine / CXCL15, TCK-1 / CXCL7, Testican 3 / SPOCK3, BAFF / TNFSF13B, GDF-7 / BMP-12, Mimecan / Osteoglycin, CD300b / LMIR5, DcTRAIL R1 / TNFRSF23, Heparan Sulfate 6-O-Sulfotransferase 3 / HS6ST3, Histone Deacetylase 8 / HDAC8, SIGNR1 / CD209b, Mast Cell Protease-11 / Prss34, Mast Cell Protease-6 / Mcpt6, Meteorin-like / METRNL, TNFRH3 / TNFRSF26
靶標(biāo)數(shù)	400

詳細(xì)描述

Introduction

RayBio^? G-Series Arrays are glass slide-based antibody arrays which allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors and other proteins from any biological fluid. Like a traditional sandwich-based ELISA, this array uses a matched pair of cytokine-specific antibodies for detection. Like the Quantibody^? Multiplex ELISA Arrays, G-Series utilizes a highly sensitive and stable fluorescent readout which can be detected by most laser fluorescent scanner systems (see below for more info). After capturing the spot densities with a laser scanner, normalization of the raw data can be easily calculated by the researcher, or by a quick copy-paste into our excel-based Analysis Tool software.

This array as well as all catalog numbers beginning with 'GS' differ from the classic G-Series Arrays in a few important ways. First, each capture antibody is printed in quadruplicate instead of duplicate, delivering higher precision. Secondly, this array features the same antibody panels used in our Quantibody Arrays, allowing a seamless transition to our quantitative multiplex assay platform. Lastly, all 16 wells are spotted as sub-arrays, delivering easy handling of 16 samples simultaneously while consuming low sample volumes (10 - 100 μl per array).

Product Features

High sensitivity (pg/mL to ng/mL levels)
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 50 μL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Test 16 samples on each slide
Data analysis software provides one-step computation

Target Names

2B4 (CD244), 4-1BB (TNFRSF9/CD137), 4-1BB Ligand (TNFSF9), 6Ckine (CCL21), ACE (CD143), ACE-2, Activin A, Activin R1B (ALK-4), ADAM15, ADAM-9, ADAMTS-1 (METH1), Adiponectin (ACRP30), ADP-Ribosyl Cyclase 2 (BST-1/CD157), ALK-1, Alpha-fetoprotein, Ameloblastin, Amphiregulin, ANGPTL1, ANGPTL3, ANGPTL7, Artemin, ASAHL, ASAM (ACAM), AXL, B7-H2 (ICOS Ligand), B7-H3 (CD276), B7-H4, BAFF, BAFF R, Betacellulin (BTC), bFGF, BLC (CXCL13), C1q R1 (CD93), C4.4A (LYPD3), C5a, CA12, CA9, Cadherin-4, Carbonic Anhydrase IV (CA4), Carbonic Anhydrase XIV (CA14), Cardiotrophin-1 (CT-1), Cathepsin H, CCL28 (MEC), CCL6, CD117 (c-kit), CD14, CD2, CD200, CD229 (SLAMF3), CD27 (TNFRSF7), CD27 Ligand (TNFSF7), CD28, CD30 (TNFRSF8), CD30 Ligand (TNFSF8), CD300b (LMIR5), CD32a, CD36 (SR-B3), CD39 (ENTPD1), CD39L3 (ENTPD3), CD4, CD40 (TNFRSF5), CD40 Ligand (TNFSF5), CD44, CD45, CD48 (SLAMF2), CD6, CD69, CD80 (B7-1), CD86 (B7-2), CD90 (Thy1), CD99-L2, CDCP1, CDNF, CEACAM-1, CFVII, Chemerin, CHL-1 (L1CAM-2), Chordin, Chordin-like 2 (CHRDL2), CLEC9A, Clusterin, COCO, Contactin-4 (BIG-2), Contactin-6, CRACC (SLAMF7), Cripto-1, CRP (C-Reactive Protein), CTLA-4 (CD152), CXADR, CXCL14 (BRAK), CXCL16, Cystatin B, Cystatin C, DAN, DcTRAIL R1 (TNFRSF23), Decorin, Dectin-2 (CLEC6A), DKK-1, DLL1, DLL4, DNAM-1 (CD226), DNER, Dtk, E-Cadherin, EDAR, EGF, Endocan, Endoglin (CD105), Endoglycan (PODXL2), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), EphA5, EphA8, EphB2, EphB4, EphB6, Ephrin-A2, Ephrin-A4, Epigen, Epimorphin (Syntaxin 2), Epiregulin, Erythropoietin R, E-Selectin, Fas (TNFRSF6/Apo-1), Fas Ligand (TNFSF6), FCRL1 (FcRH1), Fetuin A, FGF-10 (KGF-2), FGF-21, FGF-4, FGF-6, FLRG, Flt-3 (Flk-2), Flt-3 Ligand, Follistatin, Follistatin-like 1, Fractalkine (CX3CL1), Frizzled-1, Frizzled-4, Galectin-1, Galectin-3, Galectin-7, Gas 1, Gas 6, GCSF, GCSF R (CD114), GDF-3, GDF-7 (BMP-12), GFR alpha-3, GITR (TNFRSF18), GITR Ligand (TNFSF18), GM-CSF, gp130 (sgp130), GPV (CD42d), GPVI, Granzyme B, Gremlin-1, GRO alpha (CXCL1), H60, HAI-1, Heparan Sulfate 6-O-Sulfotransferase 3 (HS6ST3), HGF, HGFR, Histone Deacetylase 8 (HDAC8), I-309 (TCA-3/CCL1), ICAM-1 (CD54), IFN-gamma, IFN-gamma R1, IFN-gamma R2, IGF-1, IGF-2, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IGSF8 (CD316), IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 R1, IL-1 R2, IL-1 R4 (ST2), IL-1 R6 (IL-1 Rrp2), IL-1 Ra (IL-1 F3), IL-10, IL-10 R beta, IL-11 R alpha, IL-12 p40/p70, IL-12 p70, IL-13, IL-15, IL-15 R alpha, IL-16, IL-17 RA, IL-17 RB, IL-17 RC, IL-17A, IL-17B, IL-17C, IL-17E (IL-25), IL-17F, IL-18 BPc, IL-18 R beta (AcPL), IL-2, IL-2 R alpha, IL-20, IL-20 R beta, IL-21, IL-21 R, IL-22, IL-23, IL-23 R, IL-28A (IFN-lambda 2), IL-28A (IFN-lambda 2), IL-3, IL-3 R beta, IL-31, IL-33 (IL-1 F11), IL-34, IL-36 beta (IL-1 F8), IL-4, IL-5, IL-6, IL-7, IL-7 R alpha (CD127), IL-9, I-TAC (CXCL11), JAM-A (CD321/F11R), JAM-C (CD323), Kallikrein 7, Klotho beta, Kremen-1, Kremen-2, Laminin alpha 4, LAMP1 (CD107a), Layilin, LDL R, Leptin, Leptin R, LIF, LIGHT (TNFSF14), Limitin, LIMPII, Lipocalin-2 (NGAL), LIX, LOX-1, LRPAP, LRRC32 (GARP), L-Selectin (CD62L), Lungkine (CXCL15), Lymphotactin (XCL1), Lymphotoxin beta R (TNF RIII), MAdCAM-1, Marapsin, Mast Cell Protease-11 (Prss34), Mast Cell Protease-6 (Mcpt6), Matrilin-2, Matrilin-3, MBL-2, MCP-1 (CCL2), MCP-5, M-CSF, MDC (CCL22), MEP1A, MEPE (OF45), MESDC2, Meteorin, Meteorin-like (METRNL), MFG-E8, MIG (CXCL9), Mimecan (Osteoglycin), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 gamma, MIP-2, MIP-3 alpha (CCL20), MIP-3 beta (CCL19), MMP-10, MMP-2, MMP-3, Nectin-2 (CD112), Nephrin, Neprilysin, Neurocan, Neurturin, NGFR (TNFRSF16), NgR, NKp46 (NCR1), Nope, NOV (CCN3), Olfactomedin-1, Oncostatin M, Oncostatin M R beta (IL-31 R beta), Osteoactivin (GPNMB), Osteoadherin (Osteomodulin), Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), OX40 (TNFRSF4), OX40 Ligand (TNFSF4), P-Cadherin, PD-1, PDGF R beta, PDGF-AA, PD-L2 (B7-DC), Pentraxin-3 (TSG-14), Periostin, Persephin, PILR-beta, PLA2G2A, Platelet Factor 4 (CXCL4), Plexin A1, Plexin C1, PLGF-2, Podocalyxin, Podoplanin, Progranulin, Prolactin, Pro-MMP-9, Prostasin, Protocadherin-12, P-Selectin, RAGE, RANK (TNFRSF11A), RANTES (CCL5), Reg2, Relaxin-1, Renin 1, Resistin, Ret, RGM-B, RGM-C (Hemojuvelin), ROBO3, SCF, SDF-1 alpha (CXCL12 alpha), SEMA3F, Semaphorin 3C, Semaphorin 4C, Semaphorin 4F, Semaphorin 4G, Semaphorin 6C, sFRP-3, Siglec-3, Siglec-E, SIGNR1 (CD209b), SLAM (CD150), Slit2, SMOC-1, Sonic Hedgehog N-Terminal (Shh-N), SorCS2, SP-D, SR-AI (MSR), STC-2, Syndecan-1, Syndecan-3, TACI (TNFRSF13B), TARC (CCL17), TCK-1 (CXCL7), TECK (CCL25), Testican 3 (SPOCK3), TFPI-2, TGF beta 1, TGF-beta RI (ALK-5), TGF-beta RII, Thrombopoietin (TPO), Thrombopoietin R (Tpo R), TIGIT, TIM-1 (KIM-1), TIM-3 (KIM-3), TLR2, TNF alpha, TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TNFRH3 (TNFRSF26), TRAIL (TNFSF10), TRAIL R2 (TNFRSF10B/DR5), TRANCE (TNFSF11), Transglutaminase 2 (TGM2), TREM-1, TREM-2, TREML1 (TLT-1), TRKC, TROP1 (EpCAM), TROP-2, TROY (TNFRSF19), Trypsin 3 (PRSS3), Tryptase epsilon, Tryptase-5, TSLP, TSLP R, TWEAK (TNFSF12), TWEAK R (TNFRSF12), uPAR, VCAM-1 (CD106), VE-Cadherin (CDH5), VEGF-A, VEGF-B, VEGF-D, VEGFR1, VEGFR2, VEGFR3, Wnt-2b

Application Notes

Suggested Application

Multiplexed Protein Detection
Detection of Relative Protein Expression
Detecting Patterns of Cytokine Expression
Biomarker Screening
Identifying Key Factors
Confirming Biological Process

Kit Components

Antibody Array Slide/s
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
30 ml Centrifuge Tube
Adhesive Plastic Strips
Manual

Other Materials Required

Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Prepare Standards
Block array surface
Incubate with samples
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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文件

GSM-CAA-400.pdf

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